LOCUS       sequence-128791-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modular vector for secreted luciferase reporter expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Oliveira Paiva AM, Friggen AH, Hossein-Javaheri S, Smits WK
  TITLE     The Signal Sequence of the Abundant Extracellular Metalloprotease 
            PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in 
            Clostridium difficile.
  JOURNAL   ACS Synth Biol. 2016 Jun 23.
  PUBMED    27333161
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 23."
COMMENT     Sequence Label: pAP24
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    156..174
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt aattcctcct
       61 ttttgttgac attatatcat tgatagagtt atttgtcaaa ctagtttttt atttcgatgc
      121 cctggacttc atgaaaaact aaaaaaaata ttgacactct atcattgata gagtataatt
      181 aaaataagct tgatcgtagc gttaacagat ctgagctcct gcagtaaagg agaaaatttt
      241 atgagaccaa gtaaaaaatt attaatagct ataatatcaa tattcttaat aagttcagtt
      301 ccagtaagtg cacatgcaga tagtgttttt acacttgaag attttgtggg tgattggaga
      361 caaactgctg gatataattt agatcaagtt ttagaacagg gtggagtgag ttctcttttt
      421 caaaatcttg gtgtctcagt tacaccaata caaagaatag ttttaagtgg agaaaatgga
      481 ttaaagatcg atatacatgt aataattcct tatgagggac tatcagggga ccaaatggga
      541 caaattgaga aaatattcaa agttgtatat ccagtagacg atcatcactt taaagtaata
      601 ttacattatg gaactttagt aattgatggt gtaacaccaa atatgataga ttattttggt
      661 agaccatacg aagggattgc agtttttgat ggaaagaaaa taaccgtaac acgcactttg
      721 tggaatggaa ataaaataat agatgaaagg ttaattaatc ctgatggatc tttattgttt
      781 agagtaacta taaacggtgt tacaggttgg agactttgtg aacgtattct agcataggga
      841 tcctataagt tttaataaaa ctttaaatag aaaaaggctt ctctcatgag aagtcttttt
      901 tatttaaaat aaatataaa
//
