LOCUS       sequence-128795-       10049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MLV retroviral vector expressing the hTERT reverse transcriptase 
            immortalizing gene and a tNGFR marker for tracking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Andersen H, Barsov EV, Trivett MT, Trubey CM, Giavedoni LD, Lifson 
            JD, Ott DE, Ohlen C
  TITLE     Transduction with human telomerase reverse transcriptase 
            immortalizes a rhesus macaque CD8+ T cell clone with maintenance of 
            surface marker phenotype and function.
  JOURNAL   AIDS Res Hum Retroviruses. 2007 Mar;23(3):456-65.
  PUBMED    17411379
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AIDS Res 
            Hum Retroviruses."; date: "2007-03"; volume: "23(3)"; pages: 
            "456-65"
COMMENT     Sequence Label: xlox dNGFR-TERT
FEATURES             Location/Qualifiers
     source          1..10049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..525
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    589..930
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             997..1413
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1343..1365
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1381..1397
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1479..4877
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(4956..4979)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5345..5364
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     LTR             6333..6848
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(7017..7033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7017..7033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7030..7052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7041..7057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7065..7071,7072..7089,7090..7095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7110..7131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7248..7265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7419..8007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7499..7518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8178..8969,8970..9038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8801..8820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9039..9143)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9211..9229
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9267..9289)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9389..9408
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9602..9624
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9616..9633
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9617..9633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9836..9855
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
       61 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
      121 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
      181 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
      241 cagatgtttc cagggtgccc caaggacctg aaaatgaccc tgtgccttat ttgaactaac
      301 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
      361 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
      421 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag
      481 ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga
      541 gatttggaga cccctgccta gggaccaccg acccccccgc cgggaggtaa gctggccagc
      601 ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc
      661 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      721 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
      781 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
      841 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
      901 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
      961 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1021 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1081 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1141 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1201 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1261 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1321 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1381 ctttatccag ccctcactcc ttctctaggc gccggaatta gatcccggaa ttcgtcctgc
     1441 tgcgcacgtg ggaagccctg gccccggcca cccccgcgat gccgcgcgct ccccgctgcc
     1501 gagccgtgcg ctccctgctg cgcagccact accgcgaggt gctgccgctg gccacgttcg
     1561 tgcggcgcct ggggccccag ggctggcggc tggtgcagcg cggggacccg gcggctttcc
     1621 gcgcgctggt ggcccagtgc ctggtgtgcg tgccctggga cgcacggccg ccccccgccg
     1681 ccccctcctt ccgccaggtg tcctgcctga aggagctggt ggcccgagtg ctgcagaggc
     1741 tgtgcgagcg cggcgcgaag aacgtgctgg ccttcggctt cgcgctgctg gacggggccc
     1801 gcgggggccc ccccgaggcc ttcaccacca gcgtgcgcag ctacctgccc aacacggtga
     1861 ccgacgcact gcgggggagc ggggcgtggg ggctgctgct gcgccgcgtg ggcgacgacg
     1921 tgctggttca cctgctggca cgctgcgcgc tctttgtgct ggtggctccc agctgcgcct
     1981 accaggtgtg cgggccgccg ctgtaccagc tcggcgctgc cactcaggcc cggcccccgc
     2041 cacacgctag tggaccccga aggcgtctgg gatgcgaacg ggcctggaac catagcgtca
     2101 gggaggccgg ggtccccctg ggcctgccag ccccgggtgc gaggaggcgc gggggcagtg
     2161 ccagccgaag tctgccgttg cccaagaggc ccaggcgtgg cgctgcccct gagccggagc
     2221 ggacgcccgt tgggcagggg tcctgggccc acccgggcag gacgcgtgga ccgagtgacc
     2281 gtggtttctg tgtggtgtca cctgccagac ccgccgaaga agccacctct ttggagggtg
     2341 cgctctctgg cacgcgccac tcccacccat ccgtgggccg ccagcaccac gcgggccccc
     2401 catccacatc gcggccacca cgtccctggg acacgccttg tcccccggtg tacgccgaga
     2461 ccaagcactt cctctactcc tcaggcgaca aggagcagct gcggccctcc ttcctactca
     2521 gctctctgag gcccagcctg actggcgctc ggaggctcgt ggagaccatc tttctgggtt
     2581 ccaggccctg gatgccaggg actccccgca ggttgccccg cctgccccag cgctactggc
     2641 aaatgcggcc cctgtttctg gagctgcttg ggaaccacgc gcagtgcccc tacggggtgc
     2701 tcctcaagac gcactgcccg ctgcgagctg cggtcacccc agcagccggt gtctgtgccc
     2761 gggagaagcc ccagggctct gtggcggccc ccgaggagga ggacacagac ccccgtcgcc
     2821 tggtgcagct gctccgccag cacagcagcc cctggcaggt gtacggcttc gtgcgggcct
     2881 gcctgcgccg gctggtgccc ccaggcctct ggggctccag gcacaacgaa cgccgcttcc
     2941 tcaggaacac caagaagttc atctccctgg ggaagcatgc caagctctcg ctgcaggagc
     3001 tgacgtggaa gatgagcgtg cggggctgcg cttggctgcg caggagccca ggggttggct
     3061 gtgttccggc cgcagagcac cgtctgcgtg aggagatcct ggccaagttc ctgcactggc
     3121 tgatgagtgt gtacgtcgtc gagctgctca ggtctttctt ttatgtcacg gagaccacgt
     3181 ttcaaaagaa caggctcttt ttctaccgga agagtgtctg gagcaagttg caaagcattg
     3241 gaatcagaca gcacttgaag agggtgcagc tgcgggagct gtcggaagca gaggtcaggc
     3301 agcatcggga agccaggccc gccctgctga cgtccagact ccgcttcatc cccaagcctg
     3361 acgggctgcg gccgattgtg aacatggact acgtcgtggg agccagaacg ttccgcagag
     3421 aaaagagggc cgagcgtctc acctcgaggg tgaaggcact gttcagcgtg ctcaactacg
     3481 agcgggcgcg gcgccccggc ctcctgggcg cctctgtgct gggcctggac gatatccaca
     3541 gggcctggcg caccttcgtg ctgcgtgtgc gggcccagga cccgccgcct gagctgtact
     3601 ttgtcaaggt ggatgtgacg ggcgcgtacg acaccatccc ccaggacagg ctcacggagg
     3661 tcatcgccag catcatcaaa ccccagaaca cgtactgcgt gcgtcggtat gccgtggtcc
     3721 agaaggccgc ccatgggcac gtccgcaagg ccttcaagag ccacgtctct accttgacag
     3781 acctccagcc gtacatgcga cagttcgtgg ctcacctgca ggagaccagc ccgctgaggg
     3841 atgccgtcgt catcgagcag agctcctccc tgaatgaggc cagcagtggc ctcttcgacg
     3901 tcttcctacg cttcatgtgc caccacgccg tgcgcatcag gggcaagtcc tacgtccagt
     3961 gccaggggat cccgcagggc tccatcctct ccacgctgct ctgcagcctg tgctacggcg
     4021 acatggagaa caagctgttt gcggggattc ggcgggacgg gctgctcctg cgtttggtgg
     4081 atgatttctt gttggtgaca cctcacctca cccacgcgaa aaccttcctc aggaccctgg
     4141 tccgaggtgt ccctgagtat ggctgcgtgg tgaacttgcg gaagacagtg gtgaacttcc
     4201 ctgtagaaga cgaggccctg ggtggcacgg cttttgttca gatgccggcc cacggcctat
     4261 tcccctggtg cggcctgctg ctggataccc ggaccctgga ggtgcagagc gactactcca
     4321 gctatgcccg gacctccatc agagccagtc tcaccttcaa ccgcggcttc aaggctggga
     4381 ggaacatgcg tcgcaaactc tttggggtct tgcggctgaa gtgtcacagc ctgtttctgg
     4441 atttgcaggt gaacagcctc cagacggtgt gcaccaacat ctacaagatc ctcctgctgc
     4501 aggcgtacag gtttcacgca tgtgtgctgc agctcccatt tcatcagcaa gtttggaaga
     4561 accccacatt tttcctgcgc gtcatctctg acacggcctc cctctgctac tccatcctga
     4621 aagccaagaa cgcagggatg tcgctggggg ccaagggcgc cgccggccct ctgccctccg
     4681 aggccgtgca gtggctgtgc caccaagcat tcctgctcaa gctgactcga caccgtgtca
     4741 cctacgtgcc actcctgggg tcactcagga cagcccagac gcagctgagt cggaagctcc
     4801 cggggacgac gctgactgcc ctggaggccg cagccaaccc ggcactgccc tcagacttca
     4861 agaccatcct ggactgagtc gacccgggcg gccccggaat tccgggatct ctcgagtacc
     4921 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     4981 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtagcgc
     5041 caaccggctc cgttctttgg tggccccttc gcgccacttc tactcctccc ctagtcagga
     5101 agtttccccc agcaagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     5161 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     5221 cggccaatag cagctttgtt ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     5281 cgggggcggg ctcaggggcg ggctcagggg cgggcgggcg cccgaaggtc ctcccgaggc
     5341 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     5401 ccgggccttt cggcggccgc accatggggg caggtgccac cggccgcgcc atggacgggc
     5461 cgcgcctgct gctgttgctg cttctggggg tgtcccttgg aggtgccaag gaggcatgcc
     5521 ccacaggcct gtacacacac agcggtgagt gctgcaaagc ctgcaacctg ggcgagggtg
     5581 tggcccagcc ttgtggagcc aaccagaccg tgtgtgagcc ctgcctggac agcgtgacgt
     5641 tctccgacgt ggtgagcgcg accgagccgt gcaagccgtg caccgagtgc gtggggctcc
     5701 agagcatgtc ggcgccatgc gtggaggccg acgacgccgt gtgccgctgc gcctacggct
     5761 actaccagga tgagacgact gggcgctgcg aggcgtgccg cgtgtgcgag gcgggctcgg
     5821 gcctcgtgtt ctcctgccag gacaagcaga acaccgtgtg cgaggagtgc cccgacggca
     5881 cgtattccga cgaggccaac cacgtggacc cgtgcctgcc ctgcaccgtg tgcgaggaca
     5941 ccgagcgcca gctccgcgag tgcacacgct gggccgacgc cgagtgcgag gagatccctg
     6001 gccgttggat tacacggtcc acacccccag agggctcgga cagcacagcc cccagcaccc
     6061 aggagcctga ggcacctcca gaacaagacc tcatagccag cacggtggca ggtgtggtga
     6121 ccacagtgat gggcagctcc cagcccgtgg tgacccgagg caccaccgac aacctcatcc
     6181 ctgtctattg ctccatcctg gctgctgtgg ttgtgggtct tgtggcctac atagccttca
     6241 agaggtggaa cagctgagcg gccgcaatcg acctgcagcc aagcttatcg ataaaataaa
     6301 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     6361 agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
     6421 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
     6481 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
     6541 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaaatg
     6601 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     6661 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     6721 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     6781 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     6841 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa
     6901 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg
     6961 aaatagttca ttatggacag cgcagaagag ctggggagaa ttaattcgta atcatggtca
     7021 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7081 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     9181 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     9241 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9301 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9361 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     9421 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     9481 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9541 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9601 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tcgctctccc ttatgcgact
     9661 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     9721 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     9781 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     9841 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     9901 gtccggcgta gaggatcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     9961 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
    10021 ataaaagatt ttatttagtc tccagaaaa
//
