LOCUS       sequence-128796-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pCW57.1-4EBP1_4xAla
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        372..883
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     801..819
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(893..912)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 attggctagc gatgtccggg ggcagcagct gcagccagac tcccagccgg gctatcccca
       61 ctcgccgcgt agccctcggc gacggcgtgc agctcccgcc cggggactac agcaccgcgc
      121 ccggcggcac gctcttcagc accgcgccgg gaggaaccag aatcatctat gaccggaaat
      181 tcctgatgga gtgtcggaac gcgcctgtgg ccaaagcacc cccaaaggac ctgccaacca
      241 ttccaggggt cactagccct accagcgatg agcctcccat gcaggccagc cagagccatc
      301 tgcacagcag cccggaagat aagcgggcag gtggtgaaga gtcacagttt gagatggaca
      361 tttaaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
      421 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca
      481 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
      541 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
      601 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
      661 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc
      721 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
      781 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
      841 gtcggctccc ttcgttgacc gaatcaccga cctctctccc cagcaattca ccatgaccga
      901 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
      961 cgcggccgcg ttcgccgact acccgccacg cgccacaccg tcgatccgga acgcaaatcg
     1021 agcgggt
//
