LOCUS       sequence-128797-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal portion of GFP along with VMA intein for 
            protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-FRB-VMAc-CGFP
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..361
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             475..486
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     832..853
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttacc atggcttcta ggatcctctg gcatgagatg tggcatgaag gcctggaaga
      121 ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc
      181 cttgcatgct atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc
      241 ctatggtcga gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa
      301 tgtcaaggac ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa
      361 gactagtgtt ttgcttaacg ttctttcgaa gtgtgccggc tctaaaaaat tcaggcctgc
      421 tcccgccgct gcttttgcac gtgagtgccg cggattttat ttcgagttac aagaattgaa
      481 ggaagacgat tattatggga ttactttatc tgatgattct gatcatcagt ttttgcttgc
      541 caaccaggtt gtcgtgcaca attgcgactt caaggaggac ggcaacatcc tggggcacaa
      601 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      661 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      721 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      781 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      841 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaaca
      901 aa
//
