LOCUS       sequence-128798-         510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of alpha-bungarotoxin mRNA that can be 
            injected into embryos..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Swinburne IA, Mosaliganti KR, Green AA, Megason SG
  TITLE     Improved Long-Term Imaging of Embryos with Genetically Encoded 
            alpha-Bungarotoxin.
  JOURNAL   PLoS One. 2015 Aug 5;10(8):e0134005. doi: 
            10.1371/journal.pone.0134005. eCollection 2015.
  PUBMED    26244658
REFERENCE   2  (bases 1 to 510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-5"; volume: "10(8):e0134005. doi"; pages: " 
            10.1371/journal.pone.0134005. eCollection 2015"
COMMENT     Sequence Label: pmtb-t7-alpha-bungarotoxin
FEATURES             Location/Qualifiers
     source          1..510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(115..252,253..264)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(323..340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(323..339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(332..354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttgatatc ccgggtttaa acgcatgcag atctaatact
      121 caagtacaat tttaatggag tactttttta cttttactca agtaagattc tagccagata
      181 cttttacttt taattgagta aaattttccc taagtacttg tactttcact tgagtaaaat
      241 ttttgagtac tttttacacc tctgtcaaga accatatgcc ggtacccaat tcgccctata
      301 ggacgtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
      481 acgcgccctg tagcggcgca ttaagcgcgg
//
