LOCUS       sequence-128799-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of alpha-bungarotoxin mRNA that can be 
            injected into embryos..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Swinburne IA, Mosaliganti KR, Green AA, Megason SG
  TITLE     Improved Long-Term Imaging of Embryos with Genetically Encoded 
            alpha-Bungarotoxin.
  JOURNAL   PLoS One. 2015 Aug 5;10(8):e0134005. doi: 
            10.1371/journal.pone.0134005. eCollection 2015.
  PUBMED    26244658
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-5"; volume: "10(8):e0134005. doi"; pages: " 
            10.1371/journal.pone.0134005. eCollection 2015"
COMMENT     Sequence Label: pmtb-t7-alpha-bungarotoxin
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(56..67,68..205)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    297..431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(334..353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     388..407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(790..809)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(791..809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(817..835)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(818..835)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgcgcgtaat acgacgtcct atagggcgaa ttgggtaccg gcatatggtt cttgacagag
       61 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
      121 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
      181 tccattaaaa ttgtacttga gtattagatc tgcatgcgtt taaacccggg atatcaaaaa
      241 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      421 atgtctggat caccggttcc ggatacgtat gaattcttac tgcagatcat ccagggcgct
      481 gcttagggtg agggttgcac ttgtcggtgc tacagcaggt cacctcttcg taaggtttct
      541 tgctagggca ggtagcggcg catcccagct cgaccacctt gcctctgctg ctacagaagg
      601 cgtcgcacca catctttctg tagcacaggt tctcgccagg agggcaggtc acagcgctga
      661 tagggctggt ggcggtggtg tggcacacga tggtgtatcc caggtccagg cacacgattg
      721 tcaccaccac cagggtcagc agcagggtct tcatggtggc ggatcctgca aaaagaacaa
      781 gtagcttctc cctatagtga gtcgtattag tactcttcta tagtgtcacc taaataggcc
      841 taggatggct gaactgtaaa agaaagggaa actgttagaa ttgcactgct gccagggaga
      901 ggcgtttcag caagcatgtg accatctgga gtcaacttcc tgttttgctc tgcattctac
      961 agtctgaccg ctggtgggcg ctagtgacag cgcgcgcctc agatatgcca gacaaaggaa
     1021 gtccctctgc attctctcat tattaccata aaaggcaatg gtttgagccg ctttgcggct
     1081 gccggcggtg tggcactcag cc
//
