LOCUS       sequence-128801-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G 
            mutation along with VMA intein for protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: 3XFLAG-NSarQ27G-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..102
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gggagaccca agcttggtac cgagctcgga tccatggact acaaagacca tgacggtgat
       61 tataaagatc atgacatcga ttacaaggat gacgatgaca agcttgcggc cgcagttact
      121 tggacttgcc tgaacgacca gaaaaacccg aaaactaaca aatacgaaac taaacgtctg
      181 ctgtacaacg ggtgctttgc caagggtacc aatgttttaa tggcggatgg gtctattgaa
      241 tgtattgaaa acattgaggt tggtaataag gtcatgggta aagatggcag acctcgtgag
      301 gtaattaaat tgcccagagg aagagaaact atgtacagcg tcgtgcagaa aagtcagcac
      361 agagcccaca aaagtgactc aagtcgtgaa gtgccagaat tactcaagtt tacgtgtaat
      421 gcgacccatg agttggttgt tagaacacct cgtagtgtcc gccgtttgtc tcgtaccatt
      481 aagggtgtcg aatattttga agttattact tttgagatgg gccaaaagaa agcccccgac
      541 ggtagaattg ttgagcttgt caaggaagtt tcaaagagct acccaatatc tgaggggcct
      601 gagagagcca acgaattagt agaatcctat agaaaggctt caaataaagc ttattttgag
      661 tggactattg aggccagaga tctttctctg ttgggttccc atgttcgtaa agctacctac
      721 cagacttacg ctccaattct t
//
