LOCUS       sequence-128802-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal portion of alpha sarcin with Q27G mutation 
            along with VMA intein for protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: NSarQ27G-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccaccat ggcagttact tggacttgcc tgaacgacca
      121 gaaaaacccg aaaactaaca aatacgaaac taaacgtctg ctgtacaacg ggtgctttgc
      181 caagggtacc aatgttttaa tggcggatgg gtctattgaa tgtattgaaa acattgaggt
      241 tggtaataag gtcatgggta aagatggcag acctcgtgag gtaattaaat tgcccagagg
      301 aagagaaact atgtacagcg tcgtgcagaa aagtcagcac agagcccaca aaagtgactc
      361 aagtcgtgaa gtgccagaat tactcaagtt tacgtgtaat gcgacccatg agttggttgt
      421 tagaacacct cgtagtgtcc gccgtttgtc tcgtaccatt aagggtgtcg aatattttga
      481 agttattact tttgagatgg gccaaaagaa agcccccgac ggtagaattg ttgagcttgt
      541 caaggaagtt tcaaagagct acccaatatc tgaggggcct gagagagcca acgaattagt
      601 agaatcctat agaaaggctt caaataaagc ttattttgag tggactattg aggccagaga
      661 tctttctctg ttgggttccc atgttcgtaa agctacctac cagacttacg ctccaattct
      721 ttattctaga aacaacggca acgggaacgg cactagagga gtgcaggtgg aaaccatctc
      781 cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccg
//
