LOCUS       sequence-128809-        8317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A positive control receptor prey (rat CD200) EXPRESs plasmid for The
            Basic AVEXIS kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: CD200-Cd4d3+4-COMP-blac-3xFLAG-6his
FEATURES             Location/Qualifiers
     source          1..8317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             767..1552
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             1559..1624
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1631..1648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1686..1741
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1740..1759)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3977..3995)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4063..4167
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4168..4236,4237..5028)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5199..5787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5688..5707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5941..5958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6075..6096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6111..6116,6117..6134,6135..6141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6149..6165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6154..6176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6173..6189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6173..6189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6460..6839
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6840..7043
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6994..7014
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7040..7064
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7545..7564
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      7611..7620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggcg gcggctccgg aggcggtacc ggtggcggcg acctagcccc
      601 acagatgctt cgagaactcc aggagactaa tgcggcgctg caagacgtga gagagctctt
      661 gcgacagcag gtcaaggaga tcaccttcct gaagaatacg gtgatggaat gtgacgcctg
      721 cggtctagac agaaacctgc ctccactagc acctctgggc ccacacccag aaacgctggt
      781 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      841 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      901 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      961 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1021 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1081 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1141 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1201 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1261 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1321 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1381 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1441 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1501 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggggccctga
     1561 ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga
     1621 caagggccct caccaccacc atcaccacta attaattaag gatcccccga cctcgacctc
     1681 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     1741 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     1801 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     1861 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     1921 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     1981 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     2041 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     2101 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     2161 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     2221 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     2281 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     2341 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     2401 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     2461 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     2521 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     2581 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     2641 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     2701 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     2761 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     2821 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     2881 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     2941 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     3001 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     3061 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     3121 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     3181 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     3241 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     3301 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     3361 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     3421 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     3481 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     3541 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     3601 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     3661 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     3721 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     3781 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     3841 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     3901 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     3961 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4021 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4081 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4141 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4201 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4261 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4321 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4381 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     4441 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4501 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4561 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4621 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4681 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     4741 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4801 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4861 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4921 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4981 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5041 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5101 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5161 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5221 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5281 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5341 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5401 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5461 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5521 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5581 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5641 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5701 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5761 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5821 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5881 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5941 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6001 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6061 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6121 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6181 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     6241 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     6301 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     6361 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     6421 acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt
     6481 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     6541 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     6601 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     6661 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     6721 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     6781 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     6841 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     6901 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     6961 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     7021 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcctcact ctcttccgca
     7081 tcgctgtctg cgagggccag ctgttgggct cgcggttgag gacaaactct tcgcggtctt
     7141 tccagtactc ttggatcgga aacccgtcgg cctccgaacg gtactccgcc accgagggac
     7201 ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca
     7261 cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggtggcggtc ggggttgttt
     7321 ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc
     7381 gaggtgaggt gtggcaggct tgagatccag ctgttggggt gagtactccc tctcaaaagc
     7441 gggcattact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac
     7501 ctggcccgat ctggccatac acttgagtga caatgacatc cactttgcct ttctctccac
     7561 aggtgtccac tcccaggtcc aagtttaaac tgcggccgct ccggctagcc gccaccatgg
     7621 ccagtccggt attcaggaga cctttctgcc atctgtccac ctacagcctg ctctgggcca
     7681 tagcagcagt agcgctgagc acagctcaag tggaagtggt gacccaggat gaaagaaagc
     7741 tgctgcacac aactgcatcc ttacgctgtt ctctaaaaac aacccaggaa cccttgattg
     7801 tgacatggca gaaaaagaaa gccgtaggcc cagaaaacat ggtcacttac agcaaagccc
     7861 atggggttgt cattcagccc acctacaaag acaggataaa catcactgag ctgggactct
     7921 tgaacacaag catcaccttc tggaacacaa ccctggatga tgagggttgc tacatgtgtc
     7981 tcttcaacat gtttggatct gggaaggtct ctgggacagc ttgccttact ctctatgtac
     8041 agcccatagt acaccttcac tacaactatt ttgaagacca cctaaacatc acgtgctctg
     8101 caactgcccg cccagcccct gccatctcct ggaagggcac tgggtcagga attgagaata
     8161 gtactgagag tcactcccat tcaaatggga ctacatctgt caccagcatc ctccgggtca
     8221 aagaccccaa aactcaggtt ggaaaggaag tgatctgcca ggttttatac ttggggaatg
     8281 tgattgacta caagcagagt ctggacaaag gcgcgcc
//
