LOCUS       sequence-128810-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Bub1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC301
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             91..135
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagctgtc agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag
       61 aatctttatt ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc
      121 cagcacatgg acagcggagg tggaggtact agtctcgaga tggacacccc ggaaaatgtc
      181 cttcagatgc ttgaagccca catgcagagc tacaagggca atgaccctct tggtgaatgg
      241 gaaagataca tacagtgggt agaagagaat tttcctgaga ataaagaata cttgataact
      301 ttactagaac atttaatgaa ggaattttta gataagaaga aataccacaa tgacccaaga
      361 ttcatcagtt attgtttaaa atttgctgag tacaacagtg acctccatca attttttgag
      421 tttctgtaca accatgggat tggaaccctg tcatcccctc tgtacattgc ctgggcgggg
      481 catctggaag cccaaggaga gctgcagcat gccagtgctg tccttcagag aggaattcaa
      541 aaccaggctg aacccagaga gttcctgcaa caacaataca ggttatttca gacacgcctc
      601 actgaaaccc atttgccagc tcaagctaga acctcagaac ctctgcataa tgttcaggtt
      661 ttaaatcaaa tgataacatc aaaatcaaat ccaggaaata acatggcctg catttctaag
      721 aatcagggtt cagagctttc tggagtgata tcttcagctt gtgataaaga gtcaaatatg
      781 gaacgaagag tgatcacgat ttctaaatca gaatattctg tgcactcatc tttggcatcc
      841 aaagttgatg ttgagcaggt tgttatgtat tgcaaggaga agcttattcg tgg
//
