LOCUS       sequence-128822-         463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB224
FEATURES             Location/Qualifiers
     source          1..463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(304..325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
       61 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
      121 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
      181 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga taacagcgct
      241 ggaggctccg gtgggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      301 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      361 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      421 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcc
//
