LOCUS       sequence-128823-       10068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R->A Sensor pair 1 with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-345
FEATURES             Location/Qualifiers
     source          1..10068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     CDS             1585..1608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1609..1629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2343..2354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2467..2616
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    2635..3079
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     CDS             3110..3820
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3255..3273)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3523..3542
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3848..3969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3859..3878)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3913..3932
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4157..4745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4646..4665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4859..5162
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5277..6582
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    5619..6550
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             6598..6621
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6622..6642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    8111..8555
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        8588..8634
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        8626..8644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8654..9448
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8654..9448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8708..8727)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9318..9337
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9495..10067
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaggatc catggactac aaagacgatg acgacaagcc taagaagaaa
     1621 cggaaggtgt ccagacctgg ggaggcccca ttccagtgtc ggatctgtat ggctaatttt
     1681 agcactatgg ctgtactgag gagacataca agaacccata caggcgagaa gccctttcag
     1741 tgcaggatct gcatggcaaa cttttctcgc agagaagtgc ttgaaaacca cctgcggact
     1801 cataccggcg aaaaaccatt tcagtgtcga atttgcatgg ccaactttag ccagacagtg
     1861 aacttggata gacaccttaa gactcatctg aggcagaagg acggggaggc tcctttccag
     1921 tgcagaatct gcatggcaaa cttttctaag aaagatcatc tgcaccgcca tacccgcacc
     1981 cacaccggcg aaaaaccctt ccagtgccgc atctgtatgg ccaattttag ccagaggcca
     2041 catcttacaa atcaccttag aacacacact ggagaaaaac catttcagtg tcggatctgc
     2101 atggctaact tctccgtggg cgccagcctc aaacgccacc tgaaaaccca cctgagggga
     2161 tctgggggcg gatcaggtgg aggctcagga ggtgggtccg gaggtggatc tggtggcggg
     2221 tccggaggcg gatccgtttt gcttaacgtt ctttcgaagt gtgccggctc taaaaaattc
     2281 aggcctgctc ccgccgctgc ttttgcacgt gagtgccgcg gattttattt cgagttacaa
     2341 gaattgaagg aagacgatta ttatgggatt actttatctg atgattctga tcatcagttt
     2401 ttgcttgcca accaggttgt cgtccataat tgcaccatga ccgagaaggg cagcggaggg
     2461 cgcgccgacg cgctggacga tttcgatctc gacatgctgg gttctgatgc cctcgatgac
     2521 tttgacctgg atatgttggg aagcgacgca ttggatgact ttgatctgga catgctcggc
     2581 tccgatgctc tggacgattt cgatctcgat atgttaatta attgttaagc tagcagcagg
     2641 tttccccaat gacacaaaac gtgcaacttg aaactccgcc tggtctttcc aggtctagag
     2701 gggtaacact ttgtactgcg tttggctcca cgctcgatcc actggcgagt gttagtaaca
     2761 gcactgttgc ttcgtagcgg agcatgacgg ccgtgggaac tcctccttgg taacaaggac
     2821 ccacggggcc aaaagccacg cccacacggg cccgtcatgt gtgcaacccc agcacggcga
     2881 ctttactgcg aaacccactt taaagtgaca ttgaaactgg tacccacaca ctggtgacag
     2941 gctaaggatg cccttcaggt accccgaggt aacacgcgac actcgggatc tgagaagggg
     3001 actggggctt ctataaaagc gctcggttta aaaagcttct atgcctgaat aggtgaccgg
     3061 aggtcggcac ctttcctttg caattactga ccctatgaat acactcgaga tggtgagcaa
     3121 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     3181 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3241 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     3301 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3361 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3421 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     3481 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3541 gaccatgggc tggcaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     3601 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     3661 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     3721 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     3781 ccgccactcc accggcggca tggacgagct gtacaagtaa gcggccgcac tagctggcca
     3841 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3901 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3961 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4021 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggaaatgtt aattaactag
     4081 ccatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4801 acatgttctt gtttaaactt aatttcgctt aatttcgctc atgaaattaa cctgcaggcg
     4861 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     4921 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     4981 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     5041 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     5101 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5161 tgatgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     5221 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttgactagtg gagccgagag
     5281 taattcatac aaaaggaggg atcgccttcg caaggggaga gcccagggac cgtccctaaa
     5341 ttctcacaga cccaaatccc tgtagccgcc ccacgacagc gcgaggagca tgcgcccagg
     5401 gctgagcgcg ggtagatcag agcacacaag ctcacagtcc ccggcggtgg ggggaggggc
     5461 gcgctgagcg ggggccaggg agctggcgcg gggcaaactg ggaaagtggt gtcgtgtgct
     5521 ggctccgccc tcttcccgag ggtgggggag aacggtatat aagtgcggta gtcgccttgg
     5581 acgttctttt tcgcaacggg tttgccgtca gaacgcaggt gagtggcggg tgtggcttcc
     5641 gcgggccccg gagctggagc cctgctctga gcgggccggg ctgatatgcg agtgtcgtcc
     5701 gcagggttta gctgtgagca ttcccacttc gagtggcggg cggtgcgggg gtgagagtgc
     5761 gaggcctagc ggcaaccccg tagcctcgcc tcgtgtccgg cttgaggcct agcgtggtgt
     5821 ccgccgccgc gtgccactcc ggccgcacta tgcgtttttt gtccttgctg ccctcgattg
     5881 ccttccagca gcatgggcta acaaagggag ggtgtggggc tcactcttaa ggagcccatg
     5941 aagcttacgt tggataggaa tggaagggca ggaggggcga ctggggcccg cccgccttcg
     6001 gagcacatgt ccgacgccac ctggatgggg cgaggcctgt ggctttccga agcaatcggg
     6061 cgtgagttta gcctacctgg gccatgtggc cctagcactg ggcacggtct ggcctggcgg
     6121 tgccgcgttc ccttgcctcc caacaagggt gaggccgtcc cgcccggcac cagttgcttg
     6181 cgcggaaaga tggccgctcc cggggccctg ttgcaaggag ctcaaaatgg aggacgcggc
     6241 agcccggtgg agcgggcggg tgagtcaccc acacaaagga agagggcctt gcccctcgcc
     6301 ggccgctgct tcctgtgacc ccgtggtcta tcggccgcat agtcacctcg ggcttctctt
     6361 gagcaccgct cgtcgcggcg gggggagggg atctaatggc gttggagttt gttcacattt
     6421 ggtgggtgga gactagtcag gccagcctgg cgctggaagt cattcttgga atttgcccct
     6481 ttgagtttgg agcgaggcta attctcaagc ctcttagcgg ttcaaaggta ttttctaaac
     6541 ccgtttccag gtgttgtgaa agccaccgct aattcaaagc aatccggagt atacatggac
     6601 tacaaggacg acgatgacaa gcccaagaaa aagcgcaagg tctctagacc cggggagcgc
     6661 cccttccagt gtcgcatttg catgcggaac ttttcggata aaactaaatt gagagttcat
     6721 acccgtactc ataccggtga aaaaccgttt cagtgtcgga tctgtatgcg aaatttctcc
     6781 gttagacata atttgactag acatctacgt acgcacaccg gcgagaagcc attccaatgc
     6841 cgaatatgca tgcgcaactt cagtcaatct acttctttgc aaagacacct aaaaacccac
     6901 ctgagaggat tcggcggagt gctggagaag ggctgctttg ccaagggtac caatgtttta
     6961 atggcggatg ggtctattga atgtattgaa aacattgagg ttggtaataa ggtcatgggt
     7021 aaagatggca gacctcgtga ggtaattaaa ttgcccagag gaagagaaac tatgtacagc
     7081 gtcgtgcaga aaagtcagca cagagcccac aaaagtgact caagtcgtga agtgccagaa
     7141 ttactcaagt ttacgtgtaa tgcgacccat gagttggttg ttagaacacc tcgtagtgtc
     7201 cgccgtttgt ctcgtaccat taagggtgtc gaatattttg aagttattac ttttgagatg
     7261 ggccaaaaga aagcccccga cggtagaatt gttgagcttg tcaaggaagt ttcaaagagc
     7321 tacccaatat ctgaggggcc tgagagagcc aacgaattag tagaatccta tagaaaggct
     7381 tcaaataaag cttattttga gtggactatt gaggccagag atctttctct gttgggttgc
     7441 cacgttcgca aagccactta tcagacatac gccccaatcg gcgggggttc tgggggagga
     7501 tcaggtggtg ggtccggggg aggaagcggg ggtggctctg ggggtggatc aacacgtccc
     7561 ggggaggccc ccttccagtg caggatctgc atggctaatt ttagcattcc aaaccacctt
     7621 gcccggcaca cacggactca cacaggggag aagcccttcc agtgtagaat ttgcatggca
     7681 aacttcagcc agtcagccca cttgaaaaga cacctgcgga cccatactgg cgaaaagccc
     7741 tttcagtgcc ggatctgcat ggccaacttc tcccaggacg tttcccttgt caggcacctg
     7801 aagacacatc ttcggcagaa agatggagag gctccattcc agtgtcggat ttgtatggca
     7861 aactttagtt ctgcacaggc tttggctagg catacccgga cccacacagg tgagaagcca
     7921 ttccagtgtc gcatctgcat ggccaatttc tcccagggag gcaacctcac taggcacctg
     7981 cggactcata caggggagaa accttttcag tgtaggatct gcatggctaa tttttcacag
     8041 catccaaatt tgacgcgtca cctgaaaacc cacctgagag gatcctaaaa gcttgatggg
     8101 ggatcctagg agcaggtttc cccaatgaca caaaacgtgc aacttgaaac tccgcctggt
     8161 ctttccaggt ctagaggggt aacactttgt actgcgtttg gctccacgct cgatccactg
     8221 gcgagtgtta gtaacagcac tgttgcttcg tagcggagca tgacggccgt gggaactcct
     8281 ccttggtaac aaggacccac ggggccaaaa gccacgccca cacgggcccg tcatgtgtgc
     8341 aaccccagca cggcgacttt actgcgaaac ccactttaaa gtgacattga aactggtacc
     8401 cacacactgg tgacaggcta aggatgccct tcaggtaccc cgaggtaaca cgcgacactc
     8461 gggatctgag aaggggactg gggcttctat aaaagcgctc ggtttaaaaa gcttctatgc
     8521 ctgaataggt gaccggaggt cggcaccttt cctttgcaat tactgaccct atgaatacac
     8581 tgactgtttg acaattaatc atcggcatag tatatcggca tagtataata cgactcacta
     8641 taggagggcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     8701 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     8761 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     8821 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     8881 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     8941 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     9001 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     9061 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     9121 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     9181 gagcatgccc gacggcgagg atctcgtcgt gacacatggc gatgcctgct tgccgaatat
     9241 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     9301 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     9361 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     9421 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     9481 gcgaattcgc taggattatc cctaatacct gccaccccac tcttaatcag tggtggaaga
     9541 acggtctcag aactgtttgt ttcaattggc catttaagtt tagtagtaaa agactggtta
     9601 atgataacaa tgcatcgtaa aaccttcaga aggaaaggag aatgttttgt ggaccacttt
     9661 ggttttcttt tttgcgtgtg gcagttttaa gttattagtt tttaaaatca gtacttttta
     9721 atggaaacaa cttgaccaaa aatttgtcac agaattttga gacccattaa aaaagttaaa
     9781 tgagaaacct gtgtgttcct ttggtcaaca ccgagacatt taggtgaaag acatctaatt
     9841 ctggttttac gaatctggaa acttcttgaa aatgtaattc ttgagttaac acttctgggt
     9901 ggagaatagg gttgttttcc ccccacataa ttggaagggg aaggaatatc atttaaagct
     9961 atgggagggt tgctttgatt acaacactgg agagaaatgc agcatgttgc tgattgcctg
    10021 tcactaaaac aggccaaaaa ctgagtcctt gggttgcata gaaagctg
//
