LOCUS       sequence-128855-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expession of mCherry-LacR-PALB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-mCherry-LacR-PALB2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     47..71
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(307..325)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     575..594
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga
       61 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
      121 accgatccag cctccggact ctagcgttta aacttaagct tatggtgagc aagggcgagg
      181 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      241 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      301 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      361 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      421 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      481 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      541 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      601 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
      661 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
      721 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
      781 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
      841 ccaccggcg
//
