LOCUS       sequence-128857-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expession of FLAG-PALB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-FRT/T0-Flag-PALB2
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     47..71
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             212..235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    236..260
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga
       61 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
      121 accgatccag cctccggact ctagcgttta aacttaagct tccaccatgg actacaaaga
      181 ccatgacggt gatacatatg atcatgacat cgattacaag gatgacgatg acaagacaag
      241 tttgtacaaa aaagcaggct ccaccatgga cgagcctccc gggaagcccc tcagctgtga
      301 ggagaaggaa aagttaaagg agaaattagc attcttgaaa agggaataca gcaagacact
      361 agcccgcctt cagcgtgccc aaagagctga aaagattaag cattctatta agaaaacagt
      421 agaagaacaa gattgtttgt ctcagcagga tctctcaccg cagctaaaac actcagaacc
      481 taaaaataaa atatgtgttt atgacaagtt acacatcaaa acccatcttg atgaagaaac
      541 tggagaaaag acatctatca cacttgatgt tgggcctgag tcctttaacc ctggagatgg
      601 cccaggagga ttacctatac aaagaacaga tgacacccaa gaacattttc cccacagggt
      661 cagtgaccct agtggtgagc aaaagcagaa gctgccaagc agaagaaaga agcagcagaa
      721 gaggacattt atttcacagg agagagactg tgtctttggc actgattcac tcagattgtc
      781 tgg
//
