LOCUS       sequence-128858-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mCherry-LacR-BRCA1 siRNA resistant 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-mCherry-LacR-BRCA1
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(301..319)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     569..588
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      121 ccagcctccg gactctagcg tttaaactta agcttatggt gagcaagggc gaggaggata
      181 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      241 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      301 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      361 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      421 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      481 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      541 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      601 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      661 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      721 agaag
//
