LOCUS       sequence-128862-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Flag-BRCA1 siRNA resistant construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-FRT/T0-Flag-BRCA1
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             206..229
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    230..254
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      121 ccagcctccg gactctagcg tttaaactta agcttccacc atggactaca aagaccatga
      181 cggtgataca tatgatcatg acatcgatta caaggatgac gatgacaaga caagtttgta
      241 caaaaaagca ggcttcacca tggatttatc tgctcttcgc gttgaagaag tacaaaatgt
      301 cattaatgct atgcagaaaa tcttagagtg tcccatctgt ctggagttga tcaaggaacc
      361 tgtctccaca aagtgtgacc acatattttg caaattttgc atgctgaaac ttctcaacca
      421 gaagaaaggg ccttcacagt gtcctttatg taagaatgat ataaccaaaa ggagcctaca
      481 agaaagtacg agatttagtc aacttgttga agagctattg aaaatcattt gtgcttttca
      541 gcttgacaca ggtttggagt atgcaaacag ctataatttt gcaaaaaagg aaaataactc
      601 tcctgaacat ctaaaagatg aagtttctat catccaaagt atgggctaca gaaaccgtgc
      661 caaaagactt ctacagagtg aac
//
