LOCUS       sequence-128867-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Centrin 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCB98
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(691..735)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(748..768)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(856..877)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgcattcatt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
       61 acctctacaa atgtggtatg gctgattatg atcagttatc tagatccggt ggatcccggg
      121 cccgcggtac cgtcgactgc agaattctca gtaaaggctg gtcttcttca tgatccgaag
      181 gaactcctcc tcgttcactt cgccgtcccc atcccgatca gcttcgtcga tcatctcctg
      241 cagctcctca tccgtgaggt tctcccccag ctcgttggcc acacgcttca ggtttttgaa
      301 cgagatcttc ccggtctcat cgtcatcaaa gagcctgaag gccttcagga tttcttcttt
      361 ggtgtccttc tcggacatct tctgcgtcat cacggccagg aagtcattga agctgatctt
      421 ccccgtgcct tccctgtcca cctcggagat cattttcttc atctcttcct tcctgggttc
      481 gaagcccagc gctctcatgg ccaccttcag ctccttcgcg tcgatggtcc cacttccgtc
      541 cacgtcgaag aggtcaaatg cttcccgaac ttcttgcttc tgatcctcag tgagctcggg
      601 cttaggtgcc acctttctct tttggccggt ggaggcagcg ctgggcttct tgaagccgga
      661 agccatctcg agactagtac ctccacctcc gctgtccatg tgctggcgtt cgaatttagc
      721 agcagcggtt tctttgaatt tggcggcgcc ctgaaaataa agattctctc tagagcggcc
      781 gccaccggtg gagctccacc gatcccctcc ggacttgtac agctcgtcca tgccgagagt
      841 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttgg
//
