LOCUS       sequence-128871-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Centrin 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCB99
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(689..733)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(746..766)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
       61 ctctacaaat gtggtatggc tgattatgat cagttatcta gatccggtgg atcccgggcc
      121 cgcggtaccg tcgactgcag aattcttaat agaggctggt ctttttcatg atgcgcagga
      181 actcttgctc actgacctct ccatctccat ctcgatcagc ttcatcaatc atttcctgca
      241 gctcctcatc agtcaggttc tcacccaact ccttggccac gcgtttcaga tttttgaacg
      301 aaatcttccc agtttcatca tcatcaaaga gcttgaaagc tttcaggatt tcttctttag
      361 tatctttctc agacattttc tgggtcatca cagttaaaaa gtcaccaaag ttcatttttc
      421 ctgtcccttc cttatcaatt tcacttatca ttttcttaat ttcttctttc ttgggttcaa
      481 agcccagggc cctcattgcc accttcagtt ctttaacatc tatggtgcca gttccatccg
      541 catcgaaaag atcaaaagct tcccggatct cctgcttttg ctcttcagta agctcaggct
      601 tagggctcat tctttttcgc tgagaacttg atgccatgtt tgccttctta aagttggagg
      661 ccatctcgag actagtacct ccacctccgc tgtccatgtg ctggcgttcg aatttagcag
      721 cagcggtttc tttgaatttg gcggcgccct gaaaataaag attctctcta gagcggccgc
      781 caccggtgga gctccaccga tcccctccgg acttgtacag ctcgtccatg ccgagagtga
      841 tcccggcggc ggtcacgaac tccagcagg
//
