LOCUS       sequence-128874-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Centrin 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCB100
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(677..721)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(734..754)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
       61 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tggatcccgg
      121 gcccgcggta ccgtcgactg cagaattctt aaatgtcacc agtcataata gcaatgaact
      181 cctcttggtt tatttctcca tcaccatctt tgtcaaattc ttctatcata gctcgaagtt
      241 cttcatcact catgttttca cccaattctc tagcaacacg tcgcaaattc ctcaagctta
      301 ttttacctga atcatcatca tcaaatagtt taaatgcctt gagtatttct tcatggggat
      361 ctctttccaa tatccagtct gtcacaactt cattaaaatc ttcaaaggtg attttccctg
      421 tggcttctct gtcataatct ttaagaatct tcagtacatc agcttttttt acatcaaacc
      481 ccaaggctct cattgccacc tttaattcat gataatctat tgcttcatct ttgtctgtat
      541 caaatagttc aaaagcatct ttaatttctt gtttctgttc ctcagacagt tctcttcttt
      601 ttttcctctt tgttttgtcc actacaagct cacttctcag agctaaactc atctcgagac
      661 tagtacctcc acctccgctg tccatgtgct ggcgttcgaa tttagcagca gcggtttctt
      721 tgaatttggc ggcgccctga aaataaagat tctctctaga gcggccgcca ccggtggagc
      781 tccaccgatc ccctccggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
//
