LOCUS       sequence-128880-       13509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13509)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB408
FEATURES             Location/Qualifiers
     source          1..13509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3805..3807,3808..3810,3811..4521)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3850..3871)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4111..4130)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4458..4479
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4584..4836
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4870..4886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4870..4886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4883..4905)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4894..4910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4918..4924,4925..4942,4943..4948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4963..4984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5069..5243
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5300..6097)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5411..5430)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6021..6040
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6160..6836)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6203..6223)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7031..7055
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7576..8367
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8613..9201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9102..9121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9355..9372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9387..9527
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9453..9472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9613..9635
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9871..10065
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10131..11204)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11633..12262)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcgctggag gctccggtgg gagcatggtg agcaagggcg aggagctgtt caccggggtg
     3841 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3901 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3961 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc
     4021 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4081 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4141 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4201 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4261 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4321 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4381 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     4441 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4501 ggcatggacg agctgtacaa gtaagcttgc atgcctgcag gtcgactcta gaggatcccc
     4561 gggtaccgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     4621 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     4681 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     4741 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     4801 aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc
     4861 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4921 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4981 attaggctga attaggcgcg cctatttctg aattcaacac attgcggacg tttttaatgt
     5041 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
     5101 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     5161 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
     5221 actcacacat tattatggag aaactcgagc ttgtcgatcg actctagcta gaggatcgat
     5281 ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     5341 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     5401 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     5461 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     5521 aggcatcgcc atgtgtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     5581 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     5641 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     5701 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     5761 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     5821 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     5881 tggccagcca cgatagccgc gctgcctcgt cctggagttc attcagggca ccggacaggt
     5941 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     6001 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     6061 gagaacctgc gtgcaatcca tcttgttcaa tccccatggt cgatcgacag atctgcgaaa
     6121 gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
     6181 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     6241 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     6301 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     6361 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     6421 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     6481 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     6541 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt
     6601 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     6661 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     6721 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     6781 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     6841 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     6901 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg aattcagaaa taaattcagc
     6961 ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     7021 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     7081 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     7141 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     7201 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     7261 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     7321 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     7381 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     7441 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     7501 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     7561 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     7621 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     7681 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     7741 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     7801 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     7861 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     7921 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     7981 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     8041 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     8101 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     8161 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     8221 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     8281 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     8341 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     8401 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     8461 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     8521 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     8761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9361 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     9421 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9481 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     9541 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     9601 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     9661 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     9721 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     9781 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     9841 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     9901 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     9961 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
    10021 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
    10081 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
    10141 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
    10201 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
    10261 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
    10321 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
    10381 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
    10441 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
    10501 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
    10561 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
    10621 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
    10681 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
    10741 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
    10801 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
    10861 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
    10921 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
    10981 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
    11041 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
    11101 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
    11161 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
    11221 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
    11281 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    11341 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    11401 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    11461 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    11521 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    11581 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    11641 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    11701 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    11761 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    11821 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    11881 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    11941 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    12001 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    12061 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    12121 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    12181 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    12241 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    12301 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    12361 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    12421 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    12481 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    12541 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    12601 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    12661 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    12721 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    12781 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    12841 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    12901 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    12961 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    13021 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    13081 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    13141 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    13201 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    13261 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    13321 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    13381 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    13441 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    13501 gataaacct
//
