LOCUS       sequence-128888-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GST tagged GIV-CT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Garcia-Marcos M, Ghosh P, Ear J, Farquhar MG
  TITLE     A structural determinant that renders G alpha(i) sensitive to 
            activation by GIV/girdin is required to promote cell migration.
  JOURNAL   J Biol Chem. 2010 Apr 23;285(17):12765-77. doi: 
            10.1074/jbc.M109.045161. Epub 2010 Feb 15.
  PUBMED    20157114
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.045161"; journalName: "J Biol Chem"; date: 
            "2010-04-23- 23"; volume: "285"; issue: "17"; pages: "12765-77"
COMMENT     Sequence Label: GST-GIV-CT
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(850..872)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cctccaaaat cggatctggt tccgcgtgga tccccggaat tcggagaatt tagcctgctt
       61 catgaccatg aggcttggtc cagcagtggt agcagtccaa tccagtactt gaaaagacag
      121 accagatcaa gcccagtgct ccagcacaaa atatctgaaa cactggagag tcgacatcac
      181 aagatcaaaa ctggttcccc tggaagtgaa gttgttactc tacaacagtt tttggaagaa
      241 agcaataagc ttacctcagt acagataaag tcctcaagtc aagagaatct tttagatgaa
      301 gtaatgaaaa gtttgtctgt ctcttctgac tttttgggaa aagacaaacc agttagctgt
      361 ggtctggcca ggtcagtaag tggaaaaacc ccaggggact tctatgatag acggacaact
      421 aagcctgagt ttttgagacc tggtcctcga aaaactgaag atacctactt cattagttct
      481 gcgggaaaac ctacaccagg cactcaagga aaaataaaat tagtaaaaga atcttctctg
      541 tcacgacaat caaaagatag taacccttat gcaactttac ctcgtgcaag cagcgtgatc
      601 tcaactgccg aaggaactac acgaaggaca agcatccatg attttttgac caaggacagt
      661 agactgccta tatcagttga ttcaccacca gctgctgctg acagcaacac cactgcagca
      721 tctaatgtgg acaaagtaca agaaagcaga aattcaaaaa gcaggtctag ggagcaacaa
      781 agctcctaag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga
      841 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
      901 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggg
//
