LOCUS       sequence-128900-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses of fusion of mCherry with the N-terminal 29 amino acids of
            p63RhoGEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: p63(1-29)-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(127..837)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(405..424)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     674..692
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgctttac ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc
      181 gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac
      241 gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc
      301 gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc
      361 gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac
      421 ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc
      481 ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg
      541 ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg
      601 cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg
      661 cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc
      721 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
      781 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatggt
      841 ggcgaccggt ggatc
//
