LOCUS       sequence-128909-       12784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB128
FEATURES             Location/Qualifiers
     source          1..12784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2927..3622
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      3644..3896
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3930..3946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3930..3946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3943..3965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3954..3970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3978..3984,3985..4002,4003..4008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4023..4044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4129..4303
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4344..5369)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(5436..6111)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6306..6330
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6851..7642
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7888..8476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8377..8396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8630..8647
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8662..8802
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8728..8747)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8888..8910
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9146..9340
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9406..10479)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10908..11537)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgctgg aggctccggt gggagcatga gcgagctgat
     2941 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     3001 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     3061 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     3121 cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     3181 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     3241 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     3301 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga
     3361 gatgctgtac cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt
     3421 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa
     3481 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc
     3541 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
     3601 tagcaaactg gggcacaagt gagcttagag ctcgaatttc cccgatcgtt caaacatttg
     3661 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     3721 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     3781 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     3841 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     3901 attggttccg gaaccaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     3961 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4021 taatgagtga gctaactcac attaggctga attaggcgcg cctatttctg aattcaacac
     4081 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt
     4141 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
     4201 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct
     4261 aattccctta tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg
     4321 acagatcccg gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg
     4381 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     4441 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     4501 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     4561 ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc
     4621 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     4681 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     4741 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     4801 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     4861 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     4921 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     4981 gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca
     5041 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgaacg gcgggagatg
     5101 caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg
     5161 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
     5221 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
     5281 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
     5341 gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccggatct gcgaaagctc
     5401 gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat
     5461 gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta
     5521 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
     5581 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
     5641 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
     5701 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
     5761 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
     5821 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt
     5881 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
     5941 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
     6001 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
     6061 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
     6121 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
     6181 atgttggcaa gctgctctag ccaatacgca aaccgaattc agaaataaat tcagcctaat
     6241 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     6301 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     6361 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     6421 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     6481 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     6541 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     6601 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     6661 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     6721 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     6781 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     6841 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     6901 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     6961 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     7021 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     7081 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     7141 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     7201 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     7261 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     7321 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     7381 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     7441 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     7501 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     7561 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     7621 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     7681 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     7741 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     7801 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     8041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8521 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8581 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8641 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8701 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8761 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     8821 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8881 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     8941 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     9001 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     9061 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     9121 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     9181 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     9241 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     9301 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     9361 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     9421 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     9481 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     9541 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     9601 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     9661 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     9721 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     9781 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     9841 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     9901 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     9961 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
    10021 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
    10081 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
    10141 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
    10201 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
    10261 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
    10321 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
    10381 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
    10441 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
    10501 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
    10561 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    10621 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    10681 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    10741 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    10801 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    10861 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    10921 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    10981 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    11041 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    11101 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    11161 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    11221 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    11281 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    11341 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    11401 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    11461 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    11521 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    11581 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    11641 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    11701 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    11761 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    11821 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    11881 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    11941 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    12001 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    12061 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    12121 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    12181 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    12241 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    12301 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    12361 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    12421 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    12481 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    12541 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    12601 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    12661 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    12721 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    12781 acct
//
