LOCUS       sequence-128918-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 with fluorescent Cherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FUCas9Cherry
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      177..186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             186..251
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             258..278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
       61 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
      121 gttagacgaa gcttgggctg caggtcgact ctagaggatc tgaattctgg accggtgcca
      181 ccatggacta taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg
      241 atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag
      301 ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
      361 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
      421 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg
      481 ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
      541 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
      601 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
      661 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
      721 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
      781 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
      841 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca
      901 tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac
      961 ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc
     1021 tgattgccct gagcctgggc ctgacccc
//
