LOCUS       sequence-128920-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 with fluorescent Cherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FUCas9Cherry
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(92..802)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(370..389)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     639..657
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(803..856)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(863..910)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
       61 gattatcgat aagcttgata tcgaattgtc actacttgta cagctcgtcc atgccgccgg
      121 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
      181 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
      241 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
      301 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
      361 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
      421 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
      481 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
      541 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
      601 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
      661 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
      721 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
      781 cctcctcgcc cttgctcacc attgggccag gattctcctc gacgtcaccg catgttagca
      841 gacttcctct gccctcaccg ccctttttct tttttgcctg gccggccttt ttcgtggccg
      901 ccggcctttt gtcgcctccc agctgagaca ggtcgatccg tgtctcgtac aggccggtga
      961 tgctctggtg gatcagggtg gcgtccagca cctctttggt gctggtgtac ctcttccggt
     1021 cgatggtggt gtcaaagtac ttgaaggcgg caggg
//
