LOCUS       sequence-128922-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of guide RNA with fluorescent GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, 
            Wilcox S, Tai L, Strasser A, Herold MJ
  TITLE     An inducible lentiviral guide RNA platform enables the 
            identification of tumor-essential genes and tumor-promoting 
            mutations in vivo.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 
            10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
  PUBMED    25732831
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.02.002"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1422-32"
COMMENT     Sequence Label: FgH1tUTG
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             165..785
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             795..848
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(894..915)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
       61 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
      121 gttagacgaa gcttgggctg caggtcgact ctagaggatc caccatgtcc agactggaca
      181 agagcaaagt catcaactct gccttggagc tcctgaatga agttggcatt gagggcttga
      241 ccaccaggaa gctggcccag aagctgggtg tggagcagcc taccctgtac tggcatgtga
      301 agaacaagag ggctctgctt gatgccctgg ccattgagat gttggacagg caccacaccc
      361 acttctgccc tctggaaggg gagtcctggc aggacttcct gaggaacaat gccaagagct
      421 tcagatgtgc cttgctctcc caccgggatg gtgccaaagt tcacttgggc accaggccta
      481 cagagaagca gtatgagacc ctggagaacc agctggcatt cctgtgccaa caaggcttct
      541 ccctggaaaa tgccttgtat gccctctctg ctgtgggcca cttcaccttg ggctgtgtgc
      601 tggaggacca ggagcaccaa gttgccaagg aggagaggga gacccccacc actgactcca
      661 tgccaccact gctgcggcaa gctattgagt tgtttgacca ccaaggggct gagcctgcat
      721 tcctttttgg cctggaactg atcatctgtg gcctggaaaa gcagctgaaa tgtgagtctg
      781 gctctgggtc cggtgagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc
      841 ctggcccaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      901 agctggacgg cgacgtaaac ggccacaagt tca
//
