LOCUS       sequence-128929-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology 
            arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon 
            based on Bean Yellow Dwarf Virus; sgRNA= 1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC217
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             302..322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      333..581
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
ORIGIN
        1 gatctctgag ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc
       61 tgcttacaac aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct
      121 cttcaccctt accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga
      181 taggaagaga tacacctcta ccaaagaagt gctcgatgct accctcatcc atcagtctat
      241 cactggactc tacgagacta ggatcgatct ctcacagctc ggtggtgatt caagggctga
      301 tcctaagaag aagaggaagg tttgactcga gatatgaaga tgaagatgaa atatttggtg
      361 tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg
      421 aatttgtggc tttttctaat attaaatgaa tgtaagatca cattataatg aataaacaaa
      481 tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt
      541 atgtgctatg gtatggacta tggaatatga ttaaagataa ggagctccgg tgacggaccc
      601 atggcttcgt tgaacaacgg aaactcgact tgccttccgc acaatacatc atttcttctt
      661 agcttttttt cttcttcttc gttcatacag tttttttttg tttatcagct tacattttct
      721 tgaaccgtag ctttcgtttt cttct
//
