LOCUS       sequence-128948-       12893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12893)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB404
FEATURES             Location/Qualifiers
     source          1..12893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    334..458
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(495..500,501..518,519..525)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             579..1238
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(645..664)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1580..1885
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1764..1783
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2027..2051)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2086..3897
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3968..4220
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4254..4270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4254..4270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4267..4289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4278..4294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4302..4308,4309..4326,4327..4332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4347..4368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4453..4627
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4684..5481)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4795..4814)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5405..5424
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5544..6220)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5587..5607)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6415..6439
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6960..7751
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7997..8585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8486..8505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8739..8756
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8771..8911
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8837..8856)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8997..9019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9255..9449
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9515..10588)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11017..11646)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaat caaacaagtt tgtacaaaaa agctgaacga
      361 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      421 acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga ggggccgcat
      481 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
      541 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      601 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      661 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      721 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      781 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
      841 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      901 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      961 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1021 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1081 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1141 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1201 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
     1261 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     1321 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     1381 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     1441 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     1501 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     1561 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     1621 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     1681 ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt
     1741 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     1801 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     1861 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     1921 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagctctg ttttttatgc
     1981 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     2041 tacaaagtgg ttcgatctag aggatccccg ggtggtcagt cccttatgtt acgtcctgta
     2101 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
     2161 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
     2221 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc
     2281 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
     2341 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
     2401 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
     2461 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
     2521 ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
     2581 aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac
     2641 gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag
     2701 gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca
     2761 actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt
     2821 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
     2881 ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac
     2941 aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga
     3001 ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc
     3061 taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc
     3121 atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc
     3181 gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag
     3241 caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     3301 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc
     3361 gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat
     3421 gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg
     3481 aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg
     3541 gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac
     3601 ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat
     3661 cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt
     3721 gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt
     3781 aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa
     3841 aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc
     3901 aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac cgagctcgaa
     3961 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     4021 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     4081 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     4141 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     4201 gtcatctatg ttactagatc gggaattggt tccggaacca attcgtaatc atggtcatag
     4261 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4321 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg
     4381 cgcgcctatt tctgaattca acacattgcg gacgttttta atgtactgaa ttaacgccga
     4441 attaattcgg gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta
     4501 ttgatagaag tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca
     4561 tgagcgaaac cctataggaa ccctaattcc cttatctggg aactactcac acattattat
     4621 ggagaaactc gagcttgtcg atcgactcta gctagaggat cgatccgaac cccagagtcc
     4681 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     4741 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     4801 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     4861 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgtgt
     4921 cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
     4981 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     5041 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     5101 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     5161 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     5221 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     5281 ccgcgctgcc tcgtcctgga gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     5341 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     5401 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     5461 tccatcttgt tcaatcccca tggtcgatcg acagatctgc gaaagctcga gagagataga
     5521 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat
     5581 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     5641 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     5701 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     5761 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     5821 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     5881 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     5941 tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt
     6001 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     6061 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     6121 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     6181 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     6241 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag
     6301 ctgctctagc caatacgcaa accgaattca gaaataaatt cagcctaatt cggcgttaat
     6361 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     6421 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     6481 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     6541 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     6601 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     6661 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     6721 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     6781 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     6841 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     6901 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     6961 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     7021 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     7081 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     7141 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     7201 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     7261 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     7321 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     7381 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     7441 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     7501 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     7561 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     7621 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     7681 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     7741 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     7801 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     7861 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     7921 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8761 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8821 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     8881 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     8941 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     9001 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     9061 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     9121 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     9181 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     9241 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     9301 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     9361 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     9421 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     9481 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     9541 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     9601 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     9661 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     9721 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     9781 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     9841 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     9901 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     9961 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
    10021 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
    10081 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
    10141 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
    10201 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
    10261 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
    10321 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
    10381 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
    10441 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
    10501 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    10561 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    10621 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    10681 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    10741 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    10801 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    10861 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    10921 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    10981 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    11041 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    11101 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    11161 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    11221 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    11281 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    11341 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    11401 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    11461 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    11521 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    11581 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    11641 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    11701 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    11761 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    11821 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    11881 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    11941 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    12001 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    12061 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    12121 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    12181 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    12241 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    12301 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    12361 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    12421 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    12481 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    12541 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    12601 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    12661 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    12721 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    12781 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
    12841 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa cct
//
