LOCUS       sequence-128961-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses of fusion of mCherry with the N-terminal 29 amino acids of
            p63RhoGEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: p63(1-29)-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..174
                     /codon_start=1
                     /product="palmitoylation signal consisting of the first 29 
                     amino acids of p63RhoGEF (Goedhart et al., 2013)"
                     /label=palmitoylation signal
                     /translation="MRGGHKGGRCACPRVIRKVLAKCGCCFAR"
     regulatory      187..196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(338..356)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     606..625
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccaccatg cggggggggc acaaaggggg tcgctgtgcc
      121 tgtccccgtg tgatccgaaa agtgctggca aaatgcggct gctgcttcgc ccgggatcca
      181 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      241 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      301 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      361 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      421 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      481 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      541 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      601 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
      661 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
      721 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      781 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      841 gaacagtacg aacgcgccga gggccgccac tccaccggcg gca
//
