LOCUS       sequence-128969-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yellow Cameleon 3.60 in a clontech-style backbone for expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pYC3.60-C1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(172..684,685..687,688..690))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     365..384
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     624..645
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(748..769)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taacagttta caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      121 ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cagaattctt actcgatgtt
      181 gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac
      241 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      301 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      361 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      421 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
      481 ctgcaggccg tagcccaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      541 ggtgcagatc agcttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      601 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      661 ggtgaacagc tcctcgccct tgctcaccat gccaccgctg ccacccttgt acagctcgtc
      721 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      781 ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag
      841 cacggggccg tcgccgatgg gggtgttctg
//
