LOCUS       sequence-128973-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of mCherry with FKBP12 and the DH domain 
            (155-347) of p63RhoGEF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pmCherry-FKBP12-C1-DH
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(248..250,251..571)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacag
      241 agcggcgatg ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa
      301 gcgcggccag acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga
      361 ttcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg
      421 aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc
      481 tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct
      541 cgtcttcgat gtggagcttc taaaactgga agctcgaggc gcagcagcgg gagcaggagg
      601 agcagggtac aagtccaaga aggctctgga aaggagtatg tatgtcctga gtgaactggt
      661 agaaacagag aaaatgtacg tggacgactt ggggcagatt gtggagggtt atatggccac
      721 catggctgct cagggggtcc ccgagagtct tcgaggccgt gacaggattg tgtttgggaa
      781 tatccagcaa atctatgagt ggcaccgaga ctatttcttg caagagctac aacggtgtct
      841 gaaagatcct gattggctgg ctcagctatt catcaaacac gagcgccggc tgcatatgta
      901 tgtggtgtac tgtca
//
