LOCUS       sequence-128983-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of mCherry with FKBP12 and the DH domain 
            (155-347) of p63RhoGEF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pmCherry-FKBP12-C1-DH
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      617..626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg
      121 gtggatcccg ggcccgcggt accttagccc tcaaatcccc gcaatctccc cagcgtcatc
      181 atatcgttgc agcgcttggg cacaaagcac atgacctcca cagcttgctc taggtctgca
      241 gtatccatcc cagctctatt gtaatacttg agaaaatcct tgagcagcag ctggtatttc
      301 atgatccgct gcacaggttt gatgaggagg tcgttcagct gcaggcggtg ccccagctgc
      361 tgccggagct cctcaaagta gctgtcccca aactctgaca ccacatgctc tgacttgggc
      421 ttattctgac agtacaccac atacatatgc agccggcgct cgtgtttgat gaatagctga
      481 gccagccaat caggatcttt cagacaccgt tgtagctctt gcaagaaata gtctcggtgc
      541 cactcataga tttgctggat attcccaaac acaatcctgt cacggcctcg aagactctcg
      601 gggaccccct gagcagccat ggtggccata taaccctcca caatctgccc caagtcgtcc
      661 acgtacattt tctctgtttc taccagttca ctcaggacat acatactcct ttccagagcc
      721 ttcttggact tgtaccctgc tcctcctgct cccgctgctg cgcctcgagc ttccagtttt
      781 agaagctcca catcgaagac gagagtggca tgtggtggga tgatgcctgg gtgcccagtg
      841 gcaccatagg cataatctgg agatatagtc agtttggctc tctgacccac actcatctgg
      901 gcaacccctt c
//
