LOCUS       sequence-128986-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a Golgi located FRB domain in mammalian cells (the ECFP is
            not fluorescent).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: FRB-ECFP(W66A)-Giantin
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(592..613)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ataacagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
       61 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta
      121 tctagatccg gtggatccct atagatggcc cgtaaaacac agaatgagca ggacatgaat
      181 cattagaaag tagatggctg ctagaagtgg cactcgggtc cgtgaatgac agagtgaacg
      241 caggactcgc ttccatccaa cgccactccg ggtccttcga caactgttgc ttgtaagatc
      301 tattaacagt gcctgctcct gagtgccaca ggagccaatg ataggagtcc gggaagagtc
      361 ccattcactg tgctctaacc gtctgatctg ctcctcggcc acagagagag cattctcagc
      421 agccactctt tggtctcgtt cttcttccag cagcttcctt aattcattca cttcctgtct
      481 ggtgttgcat agctgctgct gagcttcaga aaagctttgc tgcggttctc ctcgagatct
      541 gagtccggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      601 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      661 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      721 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttggcctt
      781 gatgccgttc ttctgcttgt cggcggtgat atagacgttg tggctgatgt agttgtactc
      841 cagcttgtgc
//
