LOCUS       sequence-128989-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a Mitochondria located FRB domain in mammalian cells (the 
            ECFP is not fluorescent).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: ECFP(W66A)-FRB-MoA
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(334..612)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     primer_bind     complement(667..688)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
       61 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagttatct
      121 agatccggtg gatcctcaag accgtggcag gagcttgtat ttgtacagca caaaccccag
      181 ggcagttact gatgtggaaa atccaatgat cttcagcagg ccagaaacag agggcaggtt
      241 cctttcccag aaaccaccag cactcgaatt cgaagcttga gctcgaggac caccagtacc
      301 accagcacta ccaccagcac taccaccagc actctttgag attcgtcgga acacatgata
      361 atagaggtcc caggcttgga ggaggtcctt gacattccct gatttcatgt acttcctgca
      421 ccactcttgg gcctccatta aatctcgacc ataggcctga ttaaaggatg tttccttcag
      481 agtctggggg ccccgttcca tcatagcatg caagggctcc agcacctcaa acatgccttt
      541 cacgttcctt tccccaaagt acaaacgaga tgcctcttcc aggccttcat gccacatctc
      601 atgccagagg attccacttg ctcccttgta cagctcgtcc atgccgagag tgatcccggc
      661 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      721 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      781 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      841 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
//
