LOCUS       sequence-128991-         484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB116
FEATURES             Location/Qualifiers
     source          1..484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(103..108,109..126,127..133)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(253..272)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
       61 actatggcgg cccctcgagg ggccgcatta ggcaccccag gctttacact ttatgcttcc
      121 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      181 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      241 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      301 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      361 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      421 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      481 actg
//
