LOCUS       sequence-128998-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET sensor for Galphai1 activation, encoded on a single plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: G-beta-2A-cpV-G-gamma-2-IRES-G-alpha-i1-mTq2
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     105..126
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(229..250)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(394..447)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gggcacgggc agcttgccgg tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc
       61 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      121 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgccaccgct
      181 gccacccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      241 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
      301 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      361 gtcggcgagc tgcacgccgc cgtccatacc ggtagggccg ggattctcct ccacgtcacc
      421 gcatgttaga agacttcctc tgccctcgtt ccagatcttg aggaagctat cccaggaccc
      481 tgtcgccaca gccatgccat cgtcagtcac gcccaggcag ctgacgcggt tgtcatgccc
      541 agccaagaca cctgcccggt cggctttgag tgcatcccag acgttgcagt tgaagtcgtc
      601 gtacccagca aggaggaggc gcccgctctt ggagaaggag acagaggtga tcccgcagat
      661 gatgttgtca tgggagtaag tcatgagctc ctggtcagca cgaaggtcaa acagcctgca
      721 ggtggcgtcg tctgagccag tggcaaatgc attgccattt ggaaagaagc aaatggcatt
      781 gatgtcagac tcgtggccag tgaaggtctg ccggcacatg ccttctcgca catcccagag
      841 tttggctgaa gcatcacaag caccagagac gaacagtctg gtgtcaggag caagagaaag
      901 gctcat
//
