LOCUS       sequence-128999-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET sensor for Galphai1 activation, encoded on a single plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: G-beta-2A-cpV-G-gamma-2-IRES-G-alpha-i1-mTq2
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(545..566)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(806..825)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
       61 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
      121 tgataatatg ggctgcacac tgagcgctga ggacaaggcg gccgtggagc gcagcaagat
      181 gatcgaccgc aacctccggg aggacggaga gaaggcagcg cgcgaggtca agctgctgct
      241 gctgggtgct ggtgaatccg ggaagagcac aattgtgaag cagatgaaaa ttatccacga
      301 ggctggctac tcagaggaag agtgtaagca gtacaaagca gtggtctaca gcaacaccat
      361 ccagtccatc attgccatca ttagagctat ggggagattg aaaatcgact ttggagacgc
      421 tgctcgtgcg gatgatgctc gccaactctt cgtgcttgct ggggctgcag aggaaggctt
      481 tatgaccgcg accggttcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      541 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      601 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      661 gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg
      721 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      781 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg cc
//
