LOCUS       sequence-129000-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB133
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..30
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     270..291
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    347..471
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(614..633)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
       61 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc
      121 gaagcacatc aggccgtagc cgaaggtggt cacgagggtg ggccagggca cgggcagctt
      181 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      241 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      301 caccccggtg aacagctcct cgcccttgct caccatgctg ttatcaacca ctttgtacaa
      361 gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
      421 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggtcgacctg
      481 cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc aggttaatgg
      541 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga
      601 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca
      661 ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca
      721 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac
      781 ggctctctct tt
//
