LOCUS       sequence-129011-        6009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  humanSTARR-seq validation vector with Gateway cassette upstream of 
            the firefly luciferase gene and the SCP1 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pGL4-Gateway-SCP1
FEATURES             Location/Qualifiers
     source          1..6009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..144
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(169..174,175..192,193..199)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             253..912
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(319..338)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1254..1559
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1438..1457
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1600..1724)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1861..1870
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1867..3519
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3563..3684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3574..3593)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3628..3647
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3932..3949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4103..4691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4183..4202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4891..5682,5683..5751))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5856..5904
                     /note="synthetic polyadenylation signal"
     misc_feature    5918..6009
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5958..5977
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
       61 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      121 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      241 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      301 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      361 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      421 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      481 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      541 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      601 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      661 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      721 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      781 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      841 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      901 ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
      961 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1021 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1081 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1141 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     1201 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     1261 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     1321 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     1381 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     1441 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     1501 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     1561 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     1621 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     1681 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtagatct ccctagggta
     1741 cttatataag ggggtggggg cgcgttcgtc ctcagtcgcg atcgaacact cgagccgagc
     1801 agacgtgcct acggaccggg ctagcgacct aaagcttggc aatccggtac tgttggtaaa
     1861 gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
     1921 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
     1981 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     2041 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     2101 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     2161 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     2221 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     2281 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     2341 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     2401 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     2461 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     2521 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
     2581 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     2641 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
     2701 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     2761 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     2821 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     2881 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     2941 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     3001 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     3061 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     3121 cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     3181 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     3241 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     3301 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     3361 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     3421 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     3481 ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcggggcggc
     3541 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3601 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3661 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3721 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     3781 gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     3841 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     3901 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3961 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4021 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4081 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4141 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4201 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4261 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4321 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4381 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4441 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4501 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4561 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4621 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4681 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4741 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4801 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4861 gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat
     4921 ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac
     4981 tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg
     5041 ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag
     5101 tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt
     5161 aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt
     5221 atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt
     5281 cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt
     5341 cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct
     5401 taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt
     5461 ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac
     5521 cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa
     5581 agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag
     5641 ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca
     5701 aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct
     5761 ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag
     5821 taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac
     5881 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     5941 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     6001 catttctct
//
