LOCUS       sequence-129012-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB102
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..407)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(204..223)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
       61 ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
      121 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
      181 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
      241 atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc
      301 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac
      361 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct
      421 gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
      481 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
      541 gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
      601 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat
      661 acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc
      721 t
//
