LOCUS       sequence-129015-       13009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB110
FEATURES             Location/Qualifiers
     source          1..13009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2058..2182
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2219..2224,2225..2242,2243..2249)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2303..2962
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2369..2388)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3304..3609
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3488..3507
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3650..3774)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3785..3850
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3869..4121
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4155..4171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4155..4171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4168..4190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4179..4195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4203..4209,4210..4227,4228..4233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4248..4269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4354..4528
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4569..5594)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(5661..6336)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6531..6555
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7076..7867
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8113..8701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8602..8621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8855..8872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8887..9027
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8953..8972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9113..9135
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9371..9565
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9631..10704)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11133..11762)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaaagcgaa gggaggacgg ggaggacggt gaggccagaa gaagaaagca
      901 acagtaaaat ctcatgtgat tgcgtgcatg tcatggatct atgggatgct agtccaccgt
      961 ttggaagtat gtgcagaagg agaaaggcgc gggaggccaa aagcgacttc ggccacctct
     1021 ctactttcta gaaagtttgc tcgcagcttg taataagcgc ggaaaaggta ctcgaaaaac
     1081 cggagcaatc ggtgtggtat cagcagtcgg gttcgctaaa cctacgtctt cttattttgg
     1141 aatgttaaat tgaaagggcg tgccggccct aagcacggac gatgtgacag acggaggagt
     1201 gtgagcaatt cggatgacga tgctggccgg cagtcacgac aaagagtccg aaggggcatg
     1261 gacagggcaa tatataaatg gaaaagaggc tttgaatatt cctaaccctt cgtcgtcggc
     1321 ctcccaactt gccctcgcat gctgaattgg ccgcctatct gcgaatggcc ggccgccgac
     1381 cctaagtcgc aataacttga acggagccga tgcaaagtcc ttatcgaacg ctcgttatta
     1441 taaacggggc agacctaggt atttaattat atcaaaaccc tccgcaattt cgctaatcat
     1501 tccctaattt cttctacttt ggcgtctagt gctgtggtcg cgcttgctct caaagctcgc
     1561 tgggaggcag tggagcgtct ggcttaaaat cgatcgccgc aaccctagag ttcctttcac
     1621 ttctttctct cttgcgtgga gagcatcgac atcgaagagc tgcatcttgt cttctggtat
     1681 gcgccttgtg ccctcgtctc tgtacgtcgt tacgattgct cgatattgtg attgtctaga
     1741 cctgaaaccc gactggagct tttacgtggt gtcgattggg gcctcgagcg agtgattttt
     1801 taggttcgtt ttagccaagt acatcccact ttggtcagtc ctgttatctc ggtaaggtgt
     1861 tcagttcggt ctctggtgcg gaggtgcctc gtaagtttcc tgcatgatag atttgtctat
     1921 ccttgacttg aagaggagtc gaaggtcagc gaggttggct gtgtgctatg gacgtcggtg
     1981 ctccttggtt ccgtgcttct atgggtgttg cctcaacagt attgatgtgc ctgcagaaag
     2041 gttgactaga gttatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     2101 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     2161 cacaacatat ccagtcacta tggcggcccc tcgaggggcc gcattaggca ccccaggctt
     2221 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     2281 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     2341 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     2401 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     2461 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     2521 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     2581 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     2641 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     2701 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     2761 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2821 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2881 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2941 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     3001 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     3061 tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     3121 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     3181 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     3241 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt
     3301 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     3361 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     3421 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     3481 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     3541 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     3601 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     3661 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     3721 attgatattt atatcatttt acgtttctcg ttcaactttc ttgtacaaag tggttgataa
     3781 cagcgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3841 cgatgacaag tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     3901 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     3961 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     4021 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     4081 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg ttccggaacc
     4141 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4201 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4261 ctcacattag gctgaattag gcgcgcctat ttctgaattc aacacattgc ggacgttttt
     4321 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     4381 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     4441 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
     4501 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg
     4561 catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     4621 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     4681 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     4741 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     4801 tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     4861 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     4921 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     4981 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     5041 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     5101 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     5161 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     5221 gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt
     5281 tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc
     5341 tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
     5401 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
     5461 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
     5521 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
     5581 tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt
     5641 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
     5701 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     5761 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     5821 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     5881 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     5941 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     6001 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     6061 cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga cgtggttgga
     6121 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     6181 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     6241 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     6301 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     6361 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc
     6421 tctagccaat acgcaaaccg aattcagaaa taaattcagc ctaattcggc gttaattcag
     6481 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     6541 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     6601 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     6661 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     6721 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     6781 ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     6841 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     6901 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     6961 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     7021 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
     7081 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     7141 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     7201 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     7261 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     7321 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     7381 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     7441 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     7501 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     7561 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     7621 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     7681 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     7741 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     7801 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     7861 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     7921 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     7981 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat
     8041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8221 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     8281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8881 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     8941 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     9001 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     9061 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     9121 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     9181 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     9241 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     9301 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     9361 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     9421 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     9481 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     9541 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     9601 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     9661 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     9721 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     9781 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     9841 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     9901 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     9961 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
    10021 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
    10081 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
    10141 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
    10201 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
    10261 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
    10321 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    10381 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    10441 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    10501 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    10561 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    10621 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    10681 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
    10741 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    10801 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    10861 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    10921 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    10981 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    11041 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    11101 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    11161 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    11221 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    11281 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    11341 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    11401 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    11461 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    11521 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    11581 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    11641 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    11701 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    11761 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    11821 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    11881 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    11941 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    12001 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    12061 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    12121 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    12181 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    12241 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    12301 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    12361 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    12421 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    12481 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    12541 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    12601 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    12661 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    12721 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    12781 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    12841 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    12901 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    12961 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
