LOCUS       sequence-129021-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET sensor for Galphai2 activation, encoded on a single plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: G-beta-2A-cpV-G-gamma-2-IRES-G-alpha-i2-mTq2
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..121
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(224..245)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(389..442)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 agggcagctt gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc
       61 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      121 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatgcca ccgctgccac
      181 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      241 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt
      301 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      361 cgagctgcac gccgccgtcc ataccggtag ggccgggatt ctcctccacg tcaccgcatg
      421 ttagaagact tcctctgccc tcgttccaga tcttgaggaa gctatcccag gaccctgtcg
      481 ccacagccat gccatcgtca gtcacgccca ggcagctgac gcggttgtca tgcccagcca
      541 agacacctgc ccggtcggct ttgagtgcat cccagacgtt gcagttgaag tcgtcgtacc
      601 cagcaaggag gaggcgcccg ctcttggaga aggagacaga ggtgatcccg cagatgatgt
      661 tgtcatggga gtaagtcatg agctcctggt cagcacgaag gtcaaacagc ctgcaggtgg
      721 cgtcgtctga gccagtggca aatgcattgc catttggaaa gaagcaaatg gcattgatgt
      781 cagactcgtg gccagtgaag gtctgccggc acatgccttc tcgcacatcc cagagtttgg
      841 ctgaagcatc acaagcacca gagacgaaca gtctggtgtc aggagcaaga gaaaggctca
      901 tgacatctcc agtgtgtccg
//
