LOCUS       sequence-129022-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET sensor for Galphai2 activation, encoded on a single plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: G-beta-2A-cpV-G-gamma-2-IRES-G-alpha-i2-mTq2
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(524..545)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(785..804)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
       61 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
      121 tgataatatg ggctgcaccg tgagcgccga ggacaaggcg gcggccgagc gctctaagat
      181 gatcgacaag aacctgcggg aggacggaga gaaggcggcg cgggaggtga agttgctgct
      241 gttgggtgct ggggagtcag ggaagagcac catcgtcaag cagatgaaga tcatccacga
      301 ggatggctac tccgaggagg aatgccggca gtaccgggcg gttgtctaca gcaacaccat
      361 ccagtccatc atggccattg tcaaagccat gggcaacctg cagatcgact ttgccgaccc
      421 ctccagagcg gacgacgcca ggcagctatt tgcactgtcc tgcaccgcca ccggttctat
      481 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      541 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      601 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      661 cgtgaccacc ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
      721 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      781 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      841 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      901 gctggagtac aactacttta gcgacaacgt cta
//
