LOCUS       sequence-129034-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_773_zen_VRE_dCP
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    235..259
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(393..417)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    431..522
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(463..482)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    536..584
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccgtt tggtatgcgt cttgtgattc aaagttggct tattcaaagg atattaacga
      121 aggcagcggc acgtctgctc ggctcgaaca ttcattcaca actgatgctc tcagccaccc
      181 cgcgcccttt tataccgctg cgctcgatcc ccgggcgagc tcgatcgcag atctaccact
      241 ttgtacaaga aagctgggtc taactctcgg aggacagtac tccgaatcaa tattcgttca
      301 tataggcccc gttattggac ttcctcggag gacagtactc cgaaaactta tatcaaagaa
      361 aataggggca acaagttgcc tggcggcgcc gaagcctgct tttttgtaca aacttgtggt
      421 acctatcgat agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct
      481 agtttgtttt gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac
      541 aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc gctccaagta
      601 cctcccgtac cttaatatta cttacttatc atggtagctt gggctggcgt aatagcgaag
      661 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg
      721 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
      781 accaataggc cgaaa
//
