LOCUS       sequence-129035-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_773_zen_VRE_dCP
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(171..195)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      386..395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(458..476)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tcgataggta ccacaagttt gtacaaaaaa gcaggcttcg gcgccgccag gcaacttgtt
       61 gcccctattt tctttgatat aagttttcgg agtactgtcc tccgaggaag tccaataacg
      121 gggcctatat gaacgaatat tgattcggag tactgtcctc cgagagttag acccagcttt
      181 cttgtacaaa gtggtagatc tgcgatcgag ctcgcccggg gatcgagcgc agcggtataa
      241 aagggcgcgg ggtggctgag agcatcagtt gtgaatgaat gttcgagccg agcagacgtg
      301 ccgctgcctt cgttaatatc ctttgaataa gccaactttg aatcacaaga cgcataccaa
      361 acggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg
      421 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
      481 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
      541 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
      601 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
      661 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
      721 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
//
