LOCUS       sequence-129048-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_647_S2-1-eyg_dCP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..45
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(534..558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(821..839)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc acaagtttgt acaaaaaagc aggctccgaa ttcgcccttt
       61 gcacaatttc tgtgaagctc ggaggacagt actccggtgt caagtagctc tcgtccgcta
      121 atatcaattt agacgatata catacggcca gatctggcac gcagtctgca ttcccaatga
      181 tgcgggatgg atttggattg tggggcggaa tctctaagtg cgataagatg cggagaatta
      241 cttccgcgac ttggctccgt tttgttaaat atactatgtg tgtatgtgcg tagtacagcg
      301 acttgtacgg agtacataaa catatgcata tctgcggttt agttgtcgac attatctttt
      361 gttcgtcgtt tgaaaaatgt gtgtagttgt tggggtaact gcgcacaatt gccgggctac
      421 agggtgcttt gagtctttgt ttttggattt cgttttcgat gtttagctac gaaagccaag
      481 cgttgtgcta ttttcgaatt ttgtatttta taacccgggc aagggcgaat tcgacccagc
      541 tttcttgtac aaagtggtag atctgcgatc gagctcgccc ggggatcgag cgcagcggta
      601 taaaagggcg cggggtggct gagagcatca gttgtgaatg aatgttcgag ccgagcagac
      661 gtgccgctgc cttcgttaat atcctttgaa taagccaact ttgaatcaca agacgcatac
      721 caaacggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa acataaagaa
      781 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
      841 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
//
