LOCUS       sequence-129051-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_714_S2-1-gcm_dCP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..31
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(546..570)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      761..770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(833..851)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggtaccacaa gtttgtacaa aaaagcaggc tccgaattcg ccctttgcac aatttctgtg
       61 aagtgacagc cactgagaaa tagtgtcaag tagctctcgt ccgctaatat caatttagac
      121 gatatacata cggccagatc tggcacgcag tctgcattcc cactcggagg acagtactcc
      181 ggatttggac tcggaggaca gtactccgat ctctaagtgc gataagatct cggaggacag
      241 tactccgaat tacttccgcg acttggctcc gttttgttaa atatactatg tgtgtatgtg
      301 cgtagtacag cgacttgtac ggagtacata aacatatgca tatctgcggt ttagttgtcg
      361 acattatctt ttgttcgtcg tttgaaaaat gtgtgtagtt gttggggtaa ctgcgcacaa
      421 ttgccgggct acagggtgct ttgagtcttt gtttttggat ttcgttttcg atgtttagct
      481 acgaaagcca agcgttgtgc tattttcgaa ttttgtattt tataacccgg gcaagggcga
      541 attcgaccca gctttcttgt acaaagtggt agatctgcga tcgagctcgc ccggggatcg
      601 agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat cagttgtgaa tgaatgttcg
      661 agccgagcag acgtgccgct gccttcgtta atatcctttg aataagccaa ctttgaatca
      721 caagacgcat accaaacggc attccggtac tgttggtaaa gccaccatgg aagacgccaa
      781 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca
      841 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt t
//
