LOCUS       sequence-129055-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET sensor for Galphai3 activation, encoded on a single plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: G-beta-2A-cpV-G-gamma-2-IRES-G-alpha-i3-mTq2
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(223..244)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(388..441)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gggcagcttg ccggtggtgc agatcagctt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatgccac cgctgccacc
      181 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      241 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg
      301 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      361 gagctgcacg ccgccgtcca taccggtagg gccgggattc tcctccacgt caccgcatgt
      421 tagaagactt cctctgccct cgttccagat cttgaggaag ctatcccagg accctgtcgc
      481 cacagccatg ccatcgtcag tcacgcccag gcagctgacg cggttgtcat gcccagccaa
      541 gacacctgcc cggtcggctt tgagtgcatc ccagacgttg cagttgaagt cgtcgtaccc
      601 agcaaggagg aggcgcccgc tcttggagaa ggagacagag gtgatcccgc agatgatgtt
      661 gtcatgggag taagtcatga gctcctggtc agcacgaagg tcaaacagcc tgcaggtggc
      721 gtcgtctgag ccagtggcaa atgcattgcc atttggaaag aagcaaatgg cattgatgtc
      781 agactcgtgg ccagtgaagg tctgcc
//
