LOCUS       sequence-129056-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET sensor for Galphai3 activation, encoded on a single plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: G-beta-2A-cpV-G-gamma-2-IRES-G-alpha-i3-mTq2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(521..542)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(782..801)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
       61 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
      121 taatatgggc tgcacgttga gcgccgaaga caaggcggca gtggagcgaa gcaagatgat
      181 cgaccgcaac ttacgggagg acggggaaaa agcggccaaa gaagtgaagc tgctgctact
      241 cggtgctgga gaatctggta aaagcaccat tgtgaaacag atgaaaatca ttcatgagga
      301 tggctattca gaggatgaat gtaaacaata taaagtagtt gtctacagca atactataca
      361 gtccatcatt gcaatcataa gagccatggg acggctaaag attgactttg gggaagctgc
      421 cagggcagat gatgcccggc aattatttgt tttagctggc agtgctaccg gttctatggt
      481 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      541 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      601 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      661 gaccaccctg tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
      721 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      781 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      841 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      901 ggagtacaac
//
