LOCUS       sequence-129059-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-gamma-2, tagged with 
            circular permutated Venus (cp173V)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: cpVenus-G-gamma-2
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     569..588
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccgggccc gcggttaaag gatggcacag aaaaacttct tctccctaaa
      181 cgggttttct gaagccggaa caggggtcag gagggggtct tccttggcat gtgcttcaca
      241 gtaggccatc aaatctgcag ctgccttgga cacctttatc ctgtcgatat tggcttccat
      301 cttaagctgc tctaccagct tcctggcttg tgctatgctg gcggtgttgt tgctggccat
      361 agatctgagt ccggactcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt
      421 ctgcttgtcg gcggtgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
      481 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      541 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
      601 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      661 gtggtcgggg tagcgggcga agcactgcag gccgtagccc agggtggtca cgagggtggg
      721 ccagggcacg ggcagcttgc cggtggtgca gatcagcttc agggtcagct tgccgta
//
